BIOSENSOR CORE

Biacore® instrumentation is available for your use in the Biosensor Core Facility maintained by the Department of Physiology, University of Maryland School of Medicine. Biacore® sensor technology provides a versatile, highly sensitive, easy-to-use, and quantitative approach to study the kinetics and affinity of binding interactions under exquisitely controlled conditions. The technology relies on "surface plasmon resonance", small changes in the interaction of monochromatic light with a metallic surface that occur when a protein or other molecule binds to that surface. Many different kinds of binding reactions can be studied. Extensive sets of binding curves can be obtained robotically. User-friendly software is available for curve-fitting and extraction of kinetic constants. Many different surface chemistries are available that are compatible with a wide range of ligands and binding reactions. Ligands can be proteins, nucleic acids, lipids, or carbohydrates. The size of the..