CRAIK LAB

Determining the substrate specificity of enzymes that modify the proteome, referred to as post-translational modifying (PTM) enzymes that are central regulators of cellular signaling, is a critical step in unraveling their biological functions both in normal physiological processes and in disease states. The Craik Lab has developed a technology, known as multiplex substrate profiling by mass spectrometry (MSP-MS), to globally profile the substrate specificity of PTM enzymes. This assay uses a rationally designed, physicochemically diverse library of tetradecapeptides to provide maximum coverage of sequence space. This method has been applied to PTM enzymes to uncover biological function and guide substrate and inhibitor design with proteases and kinases from diverse sources and myriad compositions... Due to the lack of accurate, non-invasive diagnostics for pancreatic cancer, many healthy patients undergo unnecessary surgery of benign pancreatic lesions, which is costly and..

Primary location: San Francisco United States