J H

For these studies, we use an array of molecular biological, biochemical and biophysical techniques. We express and isolate membrane proteins of interest to investigate their structural properties and their function in model membranes, i.e. lipid bilayers of selected lipid and protein composition. We use a wide range of biochemical and biophysical methods, like site-directed spectroscopic labeling of membrane proteins and spectroscopy, to examine structure, kinetics, and thermodynamics of proteins. Fluorescence, electron spin resonance, or circular dichroism methods are used to examine proteins, membrane protein folding, protein-protein interactions and protein-lipid interactions. Single channel conductance recordings are used to examine the function of transmembrane proteins as transporters for solutes. We also develop new methods for these studies.

Primary location: Kassel Germany