COUMASSIE

The first time Coomassie blue was used for the visualization of proteins was in 1964. They were separated by electrophoresis, and the cellulose acetate sheet was transferred to a solution of the Coomassie dye. Two years later the same was done with proteins separated in a polyacrylamide gel. However, the Coomassie dye stained both the gel and the proteins, so the gel had to be destained afterwards by electrophoresis. Later, the destaining of the gel has been also done by using acetic acid... Subsequently, it was found that the staining of the gel can be avoided by using a colloid form of the Coomassie dye in a trichloroacetic acid solution. This way, no destaining of the gel was necessary anymore. Nowadays the typical combination of reagents used is the colloid of the G-form of the Coomassie Blue Stain, phosphoric acid, ethanol and ammonium sulfate. Alternatively, methanol and aluminium sulfate can be used... Coomassie Instant Blue is a ready-to-use, proprietorial Coomassie staining..

Also known as: Gentaur

Primary location: Bergamo Italy