RICHARD L STEVENS PHD

We identified a number of novel human and mouse MC-specific genes. We are now investigating how these genes and their transcripts are regulated as the MC's microenvironment is altered. Cis-acting elements and trans-acting DNA-binding proteins that regulate the transcription of these genes, and the RNA-binding proteins and miRNAs that regulate the stability and half-lives of their transcripts are being identified... We created transgenic mice that differ substantially in the number of MCs they have in their tissues. Other transgenic mice have been created that lack RasGRP4 and numerous MC-restricted granule mediators. A transgenic/adoptive transfer approach using newly created v‑ab l-immortalized MCs also has been developed to help evaluate the roles of MCs and their different mediators in mammals. In addition, recombinant MC proteases have been generated in order to deduce their physiologic function. Finally, what happens to T cells, epithelial cells, endothelial cells, fibroblasts,..

Primary location: Boston United States