BUSTOOLS

BUS format is a file format for single-cell RNA-seq data designed to facilitate the development of modular workflows for data processing. It consists of a binary representation of barcode and UMI sequences from scRNA-seq reads, along with sets of equivalence classes obtained by pseudoalignment of reads to a reference transcriptome (hence the acronym Barcode, UMI, Set). BUS files are a convenient and useful checkpoint during single-cell RNA-seq processing. The format is described in detail in the BUStools BUS format repository and in the preprint