YALE UNIVERSITY

3. Low signal-to-noise ratios present fundamental limits on the quality of cryo-EM results. We cover the basic principles of noise and random variables to help identify quantitatively what noise does and how its effects are best mitigated... This site is a work in progress. We're posting a set of video lectures, which contain the primary material. Our plan is to provide written notes corresponding to each video lecture, and some of these notes are available here. Finally, we will be posting the Matlab scripts we are using to make animations and simulations for the lectures... Central to the process of structure determination is the "black box" of computer processing of raw images to produce 3D density maps. We believe it important that users of cryo-EM techniques know the essential principles of operation of that black box, for two reasons.

Also known as: Cryo-EM Principles