OSMAN BILSEL

Live cell microscopy: The other question that we are interested in addressing focuses on understanding the factors leading to toxicity in live cells. To this end. we have built a 2-photon (also capable of 1-photon confocal) laser scanning microscope custom suited for long-time live cell studies. The goal is to track the spatial localization and oligomerization of SOD1 in live cells and correlate that with toxicity... Another area that we are interested in is using single-molecule fluorescence methods for studying protein folding reactions, protein-protein interactions and protein oligomer formation. Single-molecule methods can be used to probe protein oligomer formation and also, using fluorescence correlation spectroscopy, probe dynamics from the sub-microsecond to tens of seconds timescale. To enable these studies we have built... In order to probe the conformation, dynamics, oligomeric state and kinetics of biological macromolecules we have built a custom single-molecule confocal..

Primary location: Worcester United States