ELISA

31,579 Kits for Application ELISA. Enzyme-linked immunosorbent assay (ELISA) is based on an older and very similar technique called enzyme immunoassay (EIA). These immunological assays are biochemical in nature and are used to detect and quantify the presence of an antibody or an antigen in a sample. ELISA assays are applied in various fields of biotechnology and molecular biology and are being used as a diagnostic tool in medicine, as well as a quality control assay in various industries. ELISA assays allow for the detection of an unknown amount of antigen which is bound to a surface. A specific antibody is applied on the antigen bound to the surface so that it can bind to the antigen. The antibody is linked to an enzyme as reporter agent that produces a detectable signal. Often these reporters produce a light of specific wave-length (fluorescence) if expose to UV-light. The amount of antigen in the sample can be determined by comparing the light intensity to a standard of know..

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