Application of clone library analysis and real-time PCR for comparison of microbial communities in a low-grade copper sulfide ore bioheap leachate...
Microbial community of leachate from the heap bioleaching located in China were analyzed using 16S rRNA gene clone libraries and real-time quantitative PCR. Both methods indicate that Leptospirillum spp. is the dominant bacteria, and Ferroplasma acidiphilum is the only archaea detected in leachate. Clone libraries Results showed that nine operational taxonomic units (Otus) is obtained, which falls into four divisions, which Nitrospirae (74%), gamma-Proteobacteria (14%),...
Human papillomavirus detection by PCR assay in a large series of high-grade squamous intraepithelial lesions with cytohistological correlation and follow-up.