DROP-SEQ

Drop-seq is a technology we developed to enable biologists to analyze RNA expression genome-wide in thousands of individual cells at once. We first described this in a 2015 paper in Cell. Though commercial implementations of droplet-based single-cell RNA-seq also now exist, we have made Drop-seq open-source and want to make sure that any lab can build their own system. The materials for constructing a Drop-seq setup in one's own lab can be obtained for about $6,000. The reagents for performing Drop-seq cost about 6 cents per cell... Our latest software, and cookbook for core computational analysis of Drop-seq data, which shows you how to transform raw sequence data into an expression measurement for each gene in each individual cell is now open-source on GitHub. The software was developed by Jim Nemesh and Alec Wysoker in our lab. Releases can be found here... We want this web site to be a "living protocol". As people begin to adopt Drop-seq and we learn about their experiences, we..

Also known as: McCarroll Lab

Primary location: Boston United States