LITTLETON LAB

We seek to elucidate the molecular and cellular mechanisms underlying neuronal communication. We investigate the molecular machines that drive synapse formation, function and plasticity by combining genetic toolkits with electrophysiology and live imaging of neuronal activity. We leverage the power and versatility of the fruitfly Drosophila melanogaster to study glutamatergic synapses at the level of single active zones in identifiable neuronal populations... We have developed a genetically-encoded fluorescent reporter that allows us to see individual synaptic vesicles fusing at synapses... To identify neuronal dysfunctions that cause epilepsy, we have conducted behavioral screens for temperature-sensitive (TS) mutations induced by EMS that result in seizures. We have identified and characterized a glial-specific gene that is required for microdomain glial Ca 2+ oscillations. Acute disruption of this protein (termed Zydeco - a NCKX transporter), or acute induction of Ca 2+ influx..

Primary location: Cambridge United States