BIOLOGICA

A student at this stage of development, can measure glucose, which is the product of hydrolysis of sucrose , and protein that is, the enzyme catalyzing the reaction . Accordingly, we introduce the concept of the enzyme. In this case, invertase , wherein the substrate is sucrose . But the student is not yet ready to discuss about the activity as the main problem of the relationship of the enzyme [ E ] and substrate [S] is not yet clear . Basic postulate is the relationship [S] = 10km . This before we measure the activity , the student must determine Km to calculate the exact concentration of substrate , time, temperature , i.e. must simulate method determining the activity and then measured. Take the excess concentration of sucrose , add the enzyme and for a minimum period of time (time to achieve maximum detection product) , glucose is measured as a product . In the subsequent tubes , diluted sucrose (concentration of the enzyme , and the time does not change ) until it starts to..

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