SKYLINE

[Project Page, Support] Skyline is a freely-available, open-source Windows client application for building Selected Reaction Monitoring (SRM) / Multiple Reaction Monitoring (MRM), Parallel Reaction Monitoring (PRM), Data Independent Acquisition (DIA/SWATH) and DDA with MS1 quantitative methods and analyzing the resulting mass spectrometer data. Its flexible configuration supports All Molecules. It aims to employ cutting-edge technologies for creating and iteratively refining targeted methods for large-scale quantitative mass spectrometry studies in life sciences... Use prior knowledge of your proteins and peptides of interest to improve your measurement selectivity and precision... Learn more about your quantitative measurements through the rich and interactive Skyline graphical data displays.