TAY LAB

Current directions in signaling include the investigation of the mechanisms behind stochastic and digital activation, and the emergent properties of this complex signaling scheme in pathogen-host interactions and tissue inflammation. For this effort, we combine one of the most powerful arsenal of single-cell analytical tools in the world, namely high-throughput automated microfluidic cell culture, automated live cell imaging and tracking, high-throughput single-cell gene expression (qPCR) and digital-PCR, RNA sequencing, single-cell digital protein counting, and single cell multiplexed proteomics. This pipeline, applied to cells expressing fluorescent fusion proteins and reporters, can generate terabytes of dynamic single-cell data every week, with unprecedented degree of control, accuracy and reproducibility. Using these tools and modeling, we are investigating the spatio-temporal emergent properties cell signaling in cell populations and intact tissue. We are trying to understand how..

Primary location: Chicago United States