FDA SEQC

We are happy to post here the results, methods, data, scripts, and phalanx of papers for the Food and Drug Administration's (FDA) Sequencing Quality Control (SEQC) Study on RNA-Sequencing (RNA-Seq) and the Association of Biomolecular Resource Facilities (ABRF) Phase I Study on Next-Generation Sequencing (NGS), which focused on RNA-seq. These 10 complementary studies have focused on all aspects of RNA-sequencing, including: RNA quality, library preparation, sequencing, alignment, quantification, normalization, integration, and concordance. The overarching goal was to establish robust measures and methods for of inter-site and inter-platform RNA-seq concordance, which is critical for clinical applications, self-evaluation/improvement, and technology and informatics development... The third phase of the MAQC project (MAQC-III), also called Sequencing Quality Control (SEQC), aims at assessing the technical performance of next-generation sequencing platforms by generating benchmark..