PROTEASOME

42. healthy blood donors showed detectable binding of their IgGs to the cells expressing gFL, gS, and gT2C. A moderate, but statistically significant correlation was observed between plasma concentrations of anti-ADAMTS13 IgG and positive cells expressing gFL (r=0.65), gS (r=0.67), and gT2C (r=0.42). These results suggest that the microtiter-plate assay and the cell-based assay may detect differential antigenic epitopes. Moreover, antigens clustered on cell membrane may enhance antibody binding affinity, thereby increasing analytical sensitivity. Finally, our assay was able to determine kinetic changes of plasma levels of anti-ADAMTS13 IgGs in TTP individuals during plasma therapy. Collectively, our findings suggest that the novel cell-based assay may be relevant for rapid recognition and mapping of anti-ADAMTS13 autoantibodies in individuals with acquired TTP. gene 2; 2) acquired idiopathic TTP, which is mainly caused by polyclonal immunoglobulin Gs (IgGs) that inhibit plasma ADAMTS13..