LDE225

Other light-absorbing particles, such as hemoglobin or calcified structures (bones or necrotic areas), can also be easily removed by the application of Tetrakis [3] or EDTA [36] during the clearing process, respectively. Overall, it seems that TOC, in the context of SRM-based studies, offers far more advantages than limitations. and a proper combination of these might promptly reveal the three-dimensional structure of entire organs with nanometer resolution. As such, an effort to introduce large-scale volumetric SRM has already started; in this review, we discuss TOC approaches that might be favorable during the preparation of SRM samples. Thus, special emphasis is put on TOC methods that enhance the preservation of fluorescence intensity, offer the homogenous distribution of molecular probes, and vastly decrease spherical aberrations. Finally, we review examples of studies in which both SRM and TOC were successfully applied to study biological systems. with SRM precision maintained..