PROTPURI

Development of a novel carrier family and automatic method-development system for quick elaboration of recombinant proten purification technologies... Modification of novel nanoporous (15 - 50 nm) silicagels and other silica and organic polymer based solid carriers functionalized with aminoalkyl and other modifying groups, with chelating ligands. Developing standardized processes for filling the new carriers into the biologically inert columns... Optimization of the expression, fermentation and homogenization of the chosen recombinant target proteins: a novel thermostable lipase (selective biocatalysis, biodiesel production), a dUTPase (medical sensors), a phenylalanine ammonia-lyase (phenylketonuria, selective biocatalysis), a phenylalanine 2,3-aminomutase (stereoselective synthesis).