MBHA RESIN

MBHA resin is the most widely used resin for preparing peptide C-terminal amides using Boc chemistry. This resin is more HF labile than BHA resin which is important for peptides containing C-terminal Phe or Leu, since these peptides are difficult to cleave from BHA resin. The resin is sold in the HCl salt form to inhibit air oxidation of the amine function attached to the resin. After treating the resin with diisopropylethylamine/dichloromethane (DIPEA/DCM) to remove the HCl, the first amino acid can be attached to the resin using standard Boc coupling protocols... High quality MBHA resin in a range of substitution levels is available from aapptec... There are many protocols for cleaving peptides from MBHA resin. Four common protocols are standard HF, low-high HF, standard TFMSA, and low-high TFMSA. In the standard HF protocol the N-terminal Boc group is removed then the peptide-resin and a mixture of scavenger are mixed with a high concentration of HF inside a special HF apparatus...