CRISPR CITRUS

To inactivate 1,200 potantial HLB-susceptibility genes, we will use a multiplex CRISPR/Cas9 approach. Our strategy will be to include four sgRNA genes targeting four different Citrus genes in each binary vector. This multiplex approach will therefore require a minimum of 300 constructs each containing a different set of four sgRNAs, for a total of 1,200 gene knockouts in 300 Citrus plants. We intend to create and maintain four independent Citrus transgenic plants for each set of four sgRNAs, so the final plant collection created will consist of 1,200 plants housed at the Yale Plant Facility... This is meta description