MULTIPLEX IHC

Traditional immunohistology studies involve labelling a few antibodies at a time across sequential tissue sections. The power, efficiency and ultimate insights of these studies can be substantially improved by using high-plex immunofluorescence labelling on a single tissue section... Here, we present a high-content multiplex immunohistochemistry (IHC) approach that improves the throughput of current methods but maintains accessibility in set-up time and cost by using commercially available reagents, standard immunofluorescence labelling protocols and conventional widefield microscopy equipment with relatively low-cost modifications.