OPENSCOPES
Updated 889 days ago
Imperial College London, London, England SW7 2AZ, United Kingdom
openScopes aims to enable users to assemble their own low-cost but advanced capability instruments using modular components and open source software and to provide a cost-effective means to recycle or upgrade existing microscopes to the latest imaging capabilities (e.g. super resolution, 3-D imaging, high throughput...). We hope that openScopes instruments are also useful for training in advanced microscopy techniques and teaching microscopy... We have developed a wide-field open source FLIM platform based on time-gated imaging using a gated optical image intensifier. FLIM data acquisition is managed by openFLIM-GOI, a MicroManager plug-in and we provide a description of hardware components here... We have developed a low-cost modular approach to SMLM that we call easySTORM. This utilises low-cost multimode diode lasers for excitation and provides, e.g. dSTORM images, with fields of view up to 125x125 microns. It can be implemented on existing fluorescence microscopes or on our..